国兰花期调控机制研究进展

国兰花期调控技术是国兰实现产业化的关键技术之一。文章从国兰花芽分化的生殖生理基础及花芽分化后国兰发育开花的环境条件方面论述国兰花期调控机制研究的进展,总结国兰花期调控的具体措施。     

辽宁凤凰山风景区硬质景观的情感传达

介绍辽宁凤凰山风景名胜区的概况及其硬质景观的艺术设计,分析其硬质景观传达的情感和人文思想.     

不同基质含水量对秋播花椰菜工厂化育苗质量的影响

运用南京蔬菜工厂化育苗中心先进的仪器设备,经精心设计后开展试验研究,旨在探索出不同基质含水量与秋播花椰菜工厂化育苗质量间的关系。将秋季花椰菜育苗划分为播种发茅期、子叶期、1叶1心期、2～5叶期、4～5叶期5个生长阶段,在各阶段均设定4个不同的基质水分处理值进行观测比较分析。试验结果表明：秋季花椰菜育苗5个生长阶段的最佳基质含水量依次为基质最大持水量的85％、65％、65％、75％和75％;另可提供秋播花椰菜优质种苗相应标准参考值为：苗龄4～5叶,株高7．47cm,茎粗3．64mm,最大节间距o．78cm,最大叶形指数1．44。     

早熟梨不同整形方式研究

通过开展不同整形方式研究,结果表明:梨幼树不同树形对侧枝生长、树冠形成的影响不同,因此在开花结果早晚、前期产量、效益方面差异较大。以折叠式扇形和Y字形整形提高早期产量效果最为显著。在每667m2栽植110株左右的密度情况下,常常采用折叠式扇形的树形;在栽植密度大于110株/667m2的情况下,采用Y字形树形;栽植密度小于40株/667m2以下的早熟梨采用疏散分层形的树形。根据不同整形方式的方差分析,得出丰产树形结构指标:在栽培密度110株/667m2的条件下,‘翠冠’梨产量控制在3000～4000kg/667m2,枝量30000～40000枝/667m2,一般每20～25cm着生1个结果枝,叶面积系数2.5～3.2,果距保持20～25cm,留果量12000～16000个/667m2,叶果比25～30:1,枝果比2～2.5:1,中短果枝占85%～90%,单株负荷27.5～37.5kg。     

Effects of temperature,inoculum concentration,leaf age,and continuous and interrupted wetness on infection of olive plants by Spilocaea oleagina

Experiments were conducted on olive plants in controlled environments to determine the effect of conidial concentration, leaf age, temperature, continuous and interrupted leaf wetness periods, and relative humidity (RH) during the drier periods that interrupted wet periods, on olive leaf spot (OLS) severity. As inoculum concentration increased from 1·0 × 10² to 2·5 × 10⁵ conidia mL^?1, the severity of OLS increased at all five temperatures (5, 10, 15, 20 and 25°C). A simple polynomial model satisfactorily described the relationship between the inoculum concentration at the upper asymptote (maximum number of lesions) and temperature. The results showed that for the three leaf age groups tested (2–4, 6–8 and 10–12 weeks old) OLS severity decreased significantly (P < 0·001) with increasing leaf age at the time of inoculation. Overall, temperature also affected (P < 0·001) OLS severity, with the lesion numbers increasing gradually from 5°C to a maximum at 15°C, and then declining to a minimum at 25°C. When nine leaf wetness periods (0, 6, 12, 18, 24, 36, 48, 72 and 96 h) were tested at the same temperatures, the numbers of lesions increased with increasing leaf wetness period at all temperatures tested. The minimum leaf wetness periods for infection at 5, 10, 15, 20 and 25°C were 18, 12, 12, 12 and 24 h, respectively. The wet periods during early infection processes were interrupted with drying periods (0, 3, 6, 12, 18 and 24 h) at two levels of RH (70 and 100%). The length of drying period had a significant (P < 0·001) effect on disease severity, the effect depending on the RH during the interruption. High RH (100%) resulted in greater disease severity than low RH (70%). A polynomial equation with linear and quadratic terms of temperature, wetness and leaf age was developed to describe the effects of temperature, wetness and leaf age on OLS infection, which could be incorporated as a forecasting component of an integrated system for the control of OLS.     

A nested‐PCR method for rapid detection of Sclerotinia sclerotiorum on petals of oilseed rape (Brassica napus)

This study established a quick and accurate method to detect petal infection of oilseed rape (Brassica napus) by Sclerotinia sclerotiorum using a nested‐PCR technique. DNA samples were extracted from each petal using a microwave method, followed by two rounds of PCR amplification. The first‐round PCR amplification was performed using the universal fungal primer pair ITS4/ITS5, and the second‐round amplification with a specific primer pair XJJ21/XJJ222, which was designed using the single‐nucleotide polymorphisms among nuclear rDNA ITS sequences of Sclerotinia spp., Botrytis spp. and other selected fungi. The established technique is rapid and inexpensive, and has a high degree of specificity and sensitivity. This assay can distinguish Sclerotinia spp. from other fungi, including Botrytis cinerea, a closely related and frequent cohabitant on oilseed rape petals, and can detect 50 fg genomic DNA, five ascospores of S. sclerotiorumin vitro or 50 ascospores of S. sclerotiorum on one petal in approximately 6 h, even in the presence of a high background of oilseed rape DNA. This technique was successfully applied in detecting natural petal infections.     

10个小麦品种（材料）对麦长管蚜的室内苗期抗蚜性*

［目的］ 明确不同小麦品种对麦长管蚜(Sitobion avenae)的室内苗期抗性。［方法］ 在温室内采用每株小麦接种1头蚜虫的方法,测定了麦长管蚜在来源于3个国家的10个小麦品种上的发育历期（DD）、相对日均体重增长量（MRGR）和成虫日均产仔数（Rm）,以此来确定不同小麦品种（材料）的抗蚜性。［结果］ 应用多元方差分析,多目标综合判别及聚类分析等方法分析表明,麦长管蚜在‘Ww2730’、‘98 10 30’、‘Astron’上的适应性最差,这几个品种对其抗性最好,是可以利用的抗性品种（材料）;‘98 10 35’、‘98 10 32’次之;‘Batis’对麦长管蚜的适应性最好,抗性最差;‘186Tm’,‘Xanthus’,‘Amigo’对麦长管蚜适应性较好,与作为对照的‘小偃 22’一样,抗性处在中间水平。［结论］ 研究结果可以为进一步的抗蚜育种提供依据。     

辽宁省辣椒土传病害镰刀菌鉴定及rDNA-ITS序列分析

［目的］ 对分离的辣椒镰刀菌形态、基因序列进行研究,以确定病原菌的种类及这些菌的同源性,为进一步开展该病害诊断和防治研究奠定基础。［方法］ 对采自辽宁省各地辣椒土传病害的16株病菌进行形态观察及rDNA-ITS序列分析。［结果］ 引起辽宁省辣椒土传病害的主要镰刀菌有尖镰孢、茄镰孢、锐顶镰孢和串珠镰孢等4种,根据菌株分生孢子形态和rDNA ITS序列同源性,将ZW13、LY3、PJLJ、HC5、CY2、CY5、TLLJ、LY5、LY1菌株聚类为尖镰孢;FSLJ2和FSLJ3菌株聚类为茄镰孢;ZW和ZW18菌株聚类为锐顶镰孢;KPLJ、LZLJ、FSLJ1菌株聚类为串珠镰孢。［结论］ 从分子系统树和菌株间的遗传距离看出镰刀菌同种同源性都很高,不同种之间同源性相对较低,种间遗传差异较大。     

农药有效利用率与喷雾技术优化

提高农药的有效利用率是植保工作者非常关心的问题。本文阐述了农药有效利用率的广义和狭义涵义,并分析了农药使用中存在的药剂浪费、有效利用率低的问题。根据喷雾技术中的“剂量传递”过程,分析了农药有效利用率的狭义涵义,在春季果园和作物苗期,常规喷雾法的农药有效利用率只有20%~30%;在夏秋季果园和作物中后期,随着作物叶面积系数（LAI）的增加,农药的有效利用率可达到50%~60%。论文分析了造成农药有效利用率低的原因,提出喷雾技术的优化措施：（1）大田喷雾时采用机动喷杆喷雾替代背负式手动常规喷雾,可以改善雾滴沉积分布的均匀性;（2）添加喷雾助剂可以提高药液在靶标表面的润湿性;（3）优化雾滴粒径,采用细雾滴替代粗雾滴可以提高雾滴的中靶率;（4）降低施药液量可以减少药液流失;（5）加装挡板可以减少雾滴飘失等。通过以上技术的优化,可以大幅度提高农药的有效利用率,达到减量增效的目的。     

抗虫材料为主体的集成技术对稻飞虱的防控效果

本文研究了由新的抗虫材料‘中9A 07/WH1462’与控氮、晒田和天敌保护等措施有机结合的环境友好集成技术体系对稻飞虱的控制效果。结果表明,该技术体系在未施用化学农药的情况下,使稻飞虱等害虫种群数量得到有效控制,较好地保护了天敌,使稻田节肢动物群落结构得到一定优化。集成技术稻田的害虫所占比例比对照田降低了57.6%,其天敌所占比例比对照田增加了19.6%,同时集成技术稻田的水稻产量比对照田增加了10.9%。